Vermeulen Group

Michiel Vermeulen: Proteomics and Chromatin Biology


Michiel Vermeulen
Tel.: 088 75 50829/68086
Fax: 088 75 68101
Email: M.Vermeulen-3 - at - umcutrecht.nl

Vermeulen Group pictures 2009

Vermeulen Group photo 2013

About the Group leader

 

The development of an adult organism from a single fertilized egg is accompanied by the generation of some 300 different cell types.  Each of these cell types expresses a specific subset of genes in a highly regulated manner. During cellular differentiation, the genome in every cell type remains unchanged, which raises one key question: How does a single genotype give rise to such a large diversity of phenotypes? At least part of the answer to this question is epigenetics. Epigenetics is defined as changes in gene expression and phenotype that are independent of the underlying DNA sequence. In higher eukaryotes this is mainly achieved through methylation of DNA on cytosines and by post-translational modifications of histones. These epigenetic modification patterns are dynamically being established, maintained and removed from the genome during differentiation and they help to create cell-type-specific gene expression profiles. Regulatory proteins can be recruited to these modifications and exert their function at the site of recruitment. The specific binding of these so-called chromatin ‘readers’ therefore significantly contributes to the biological function of each individual epigenetic modification. Our lab is using state-of-the-art quantitative mass-spectrometry based proteomics technology to identify chromatin readers for epigenetic histone and DNA modifications. We characterize the (dynamic) complexes that these readers form, we study their biology in (differentiated) stem cells and in different model organisms and we investigate their potential deregulation in cancer.

 

Current main projects include:

 
 

Figure: Dynamic readers for 5-(hydroxy)methylcytosine and its oxidized derivatives

This figure shows a selection of identified ‘readers’ for methylcytosine (mC reader, top) and hydroxymethylcytosine (hmC reader, bottom) in mouse embryonic stem cells, neuronal progenitor cells and adult mouse brain tissue. Interactions with methylcytosine and hydroxymethylcytosine are highly dynamic during development and show only a limited overlap. For more information please refer to Spruijt et al., Cell. 2013 Feb 28;152(5):1146-59.