Timmers Group

Selected papers Chromatin & Transcription Group

 

Maria J.E. Koster, H.Th. Marc Timmers, Berend Snel (2015) Genesis of chromatin and transcription dynamics in the origin of species. Cell 161:724-36. click here to find a pdf version

Maria J.E. Koster, H.Th. Marc Timmers (2015) Regulation of anti-sense transcription by Mot1p and NC2 via removal of TATA-binding protein (TBP) from the 3'-end of genes. Nucleic Acids Res. 43:143-52. click here to find a pdf version

Koen M.A. Dreijerink, Elfi B. Conemans, Chantal Cornellissen, Marja T. van Blokland, Michael Mannstadt, Carla R. Pieterman, C. Binquet, O.M. Dekkers, M.L. Drent, A.N. van der Horst-Schrivers, Wouter W. de Herder, Bas Havekes, Eric Fliers, Ad R. Hermus, Menno R. Vriens, H.Th. Marc Timmers, Elske van der Wall, Myles Brown, Rob B. van der Luijt, Paul J. van Diest, Pierre Goudet, John R. Burgess, Gerlof D. Valk (2014) Breast-cancer predisposition in multiple endocrine neoplasia type 1. N Engl J Med. 371:583-4. click here to find a pdf version

Maria J.E. Koster, Asli D. Yildirim, P. Anthony Weil, Frank C.P. Holstege, H.Th. Marc Timmers (2014) Suppression of intragenic transcription requires the MOT1 and NC2 regulators of TATA-binding protein Nucleic Acids Res. 42:4220-9. click here to find a pdf version

Carolina R. Pieterman, Elfi B. Conemans, Koen M.A. Dreijerink, Joanne M. de Laat, H.Th.Marc Timmers, Menno R. Vriens, Gerlof D. Valk (2014) Thoracic and duodenopancreatic neuroendocrine tumors in multiple endocrine neoplasia type 1  Endocr Relat Cancer 21:R121-42. click here to find a pdf version

Roy Baas, Daphne Lelieveld, Hetty A.A.M. van Teeffelen, Philip Lijnzaad, Bas Castelijns, Frederik M.A. van Schaik, Michiel Vermeulen, David A. Egan, H.Th. Marc Timmers, Petra de Graaf (2014) A Novel Microscopy-Based High-Throughput Screening Method to Identify Proteins That Regulate Global Histone Modification Levels J Biomol Screen 19:287-296. click here to find a pdf version

Rick van Nuland, Andrea W. Schram, Frederik M.A. van Schaik, Pascal W.T.C. Jansen, Michiel Vermeulen, H.Th.Marc Timmers, (2013) Multivalent Engagement of TFIID to Nucleosomes PloS One 8:e73495. click here to find a pdf version

Andrea W. Schram, Roy Baas, Pascal W.T.C. Jansen, Anne Riss, Lazlo Tora, Michiel Vermeulen, H.Th.Marc Timmers, (2013) A Dual Role for SAGA-Associated Factor 29 (SGF29) in ER Stress Survival by Coordination of Both Histone H3 Acetylation and Histone H3 Lysine-4 Trimethylation PLoS One 8:e70035. click here to find a pdf version 

Rick van Nuland, Frederik M.A. van Schaik, Marieke Simonis, Sebastiaan van Heesch, Edwin Cuppen, Rolf Boelens, H.Th. Marc Timmers, Hugo van Ingen (2013) Nucleosomal DNA binding drives the recognition of H3K36-methylated nucleosomes by the PSIP1-PWWP domain. Epigenetics Chromatin. 6:12. click here to find a pdf version

Daniel Kofink, Marco P.M. Boks, H.Th. Marc Timmers, Martien J.H. Kas (2013) Epigenetic dynamics in psychiatric disorders: environmental programming of neurodevelopmental processes. Neurosci Biobehav Rev. 37:831-45. click here to find a pdf version

Rick van Nuland, Arne H. Smits, Paschalina Pallaki, Pascal W.T.C. Jansen, Michiel Vermeulen, H.Th. Marc Timmers (2013) Quantitative dissection and stoichiometry determination of the human SET1/MLL histone methyltransferase complexes. Mol Cell Biol. 33:2067-77. click here to find a pdf version

Nikolay S. Outchkourov, Jose M. Muino, Kerstin Kaufmann, Wilfred F. van IJcken, Marian J. Groot Koerkamp, Dik van Leenen, Petra de Graaf, Frank C. Holstege, Frank G. Grosveld, H.Th. Marc Timmers (2013) Balancing histone H3K4 methylation states by the Kdm5c/SMCX histone demethylase modulates promoter and enhancer function. Cell Reports 3:1071. click here to find a pdf version

W.W.M. Pim Pijnappel, Daniel Esch, Marijke P.A. Baltissen, Guangming Wu, Nikolai Mischerikow, Atze J. Bergsma, Erik van der Wal, Dong Wook Han Hermann vom Bruch, Sören Moritz, Phillip Lijnzaad, A.F. Maarten Altelaar, Katrin Sameith, Holm Zaehres, Albert J. R. Heck, Frank C.P. Holstege, Hans R. Schöler and H.Th. Marc Timmers (2013) A central role for TFIID in the pluripotent transcription circuitry. Nature 495: 516 click here to find the PDF version

A.F. Maarten Altelaar, Christian K. Frese, Christian Preisinger, Marco L. Hennrich, Andree W. Schram, H.Th. Marc Timmers, Albert J.R. Heck, Shabaz Mohammed (2012) Benchmarking stable isotope labeling based quantitative proteomics. J Prot 10.009 click here to find the PDF version

Sjoerd J.L. van Wijk, Adrien S.J. Melquiond, Sjoerd J. de Vries, H.Th. Marc Timmers and Alexandre M.J.J. Bonvin(2012) Dynamic Control of Selectivity in the Ubiquitination Pathway Revealed by an ASP to GLU Substitution in an Intra-molecular Salt-bridge Network. PloSCompBiol. 8:e1002754 click here to find a pdf version

Markus A. Kleinschmidt, Petra de Graaf, Hetty A.A.M. van Teeffelen and H.T.Marc Timmers (2012) Cell cycle regulation by the PRMT6 arginine methyltransferase through repression of cyclin-dependent kinase inhibitors. PLoS ONE 7:e41446. click here to find a pdf version

Gianpiero Spedale, H.Th.Marc Timmers, W.W.M. Pim Pijnappel (2012) ATAC-king the complexity of SAGA during evolution. Genes & Dev. 26:527-541. click here to find a pdf version

Mohamed-Amin Choukrallah, Dominique Kobi, Igor Martianov, W.W.M. Pim Pijnappel, Nikolai Mischerikov, Tao Ye, Albert J.R. Heck, H.Th.Marc Timmers and Irwin Davidson (2012) Interconversion between active and inactive TATA-binding protein transcription complexes in the mouse genome Nucleic Acids Res. 40:1446-1459. click here to find a pdf version

Gianpiero Spedale, Claartje A. Meddens, Maria J.E. Koster, Cheuk W. Ko, Sander R. van Hooff, Frank C.P. Holstege, H.Th.Marc Timmers, W.W.M. Pim Pijnappel (2012) Tight cooperation between Mot1p and NC2ß in regulating genome-wide transcription, repression of transcription following heat shock induction, and genetic interaction with SAGA Nucleic Acids Res. 40:996-1008. click here to find the pdf version

Carolien Wansleeben, Léon van Gurp, Petra de Graaf, Florence Mousson, H.Th. Marc Timmers and Frits Meijlink (2011) An ENU-induced point mutation in the mouse Btaf1 gene causes post-gastrulation embryonic lethality and protein instability. Mech. Dev. 28:279-88. click here to find a pdf version

Heike Sandler, Jochen Kreth, H.Th. Marc Timmers and Georg Stoecklin (2011) Not1 mediates recruitment of the deadenylase Caf1 to mRNAs targeted for degradation by tristetraprolin. Nucleic Acids Res. 39:4373-86. click here to find a pdf version

Andreas O Helbig, Sara Rosati, Pim W.W.M. Pijnappel, Bas van Breukelen, H.Th. Marc Timmers, Shabaz Mohammed, Monique Slijper and Albert J.R. Heck (2010) Perturbation of the yeast N-acetyltransferase NatB induces elevation of protein phosphorylation levels. BMC Genomics 11:685. click here to find a pdf version

Radhika A. Varier and H.Th.Marc Timmers (2011) Histone lysine methylation and demethylation pathways in cancer. BBA 1815:75-89. click here to find a pdf version

Radhika A. Varier, Nikolay S. Outchkourov, Petra de Graaf, Frederik M.A. van Schaik, Henk Jan Ensing, Fangwei Wang, Jonathan M. G. Higgins, Geert J.P.L. Kops and H.Th. Marc Timmers (2010) A phospho/methyl switch at histone H3 regulates TFIID association with mitotic chromosomes. EMBO J. 29:3967-3978 click here to find the pdf version

H.Th. Marc Timmers and Michiel Vermeulen (2010) Grasping trimethylation of histone H3 at lysine 4. Epigenomics 2:395-406. click here to find a pdf version

Jacques Bonnet, Ying-Hui Wang, Gianpiero Spedale, R. Andrew Atkinson, Christophe Romier, Ali Hamiche, W.W.M. Pim Pijnappel, H.Th. Marc Timmers, Lazlo Tora, Didier Devys and Bruno Kieffer (2010) The structural plasticity of SCA7 domains defines their differential nucleosome-binding properties. EMBO Rep. 11:612-618. click here to find a pdf version

Gianpiero Spedale, Nikolai Mischerikow, Albert J. Heck, H.Th. Marc Timmers and W.W.M. Pim Pijnappel (2010) Identification of Pep4p as the protease responsible for formation of the SAGA-related SLIK protein complex. J. Biol. Chem. 285:22793-22799. click here to find a pdf version

Petra de Graaf, Florence Mousson, Bart Geverts, Elisabeth Scheer, Laszlo Tora, Adriaan Houtsmuller, and H.Th. Marc Timmers (2010) Chromatin interaction of TATA-binding protein (TBP) is dynamically regulated in human cells. Journal of Cell Science 123:2663-2671. click here to find a pdf version.

Klaus Fortschegger, Petra de Graaf, Nikolay S. Outchkourov, Frederik M.A. van Schaik, H.Th. Marc Timmers and Ramin Shiekhattar (2010) PHF8 targets histone methylation and RNA polymerase II to activate transcription. Mol. Cell. Biol. 13:3286-3298. click here to find a pdf version.

Nasun Hah, Annemieke Kolkman, Donald D. Ruhl, W.W.M. Pim Pijnappel, Albert J.R. Heck, H.Th. Marc Timmers and W. Lee Kraus (2010) A role for BAF57 in cell cycle-dependent transcriptional regulation by the SWI/SNF chromatin remodeling complex. Cancer Research 70:4402-4411. click here to find a pdf version

Frank Johannes, RenéWardenaar, Maria Colomé-Tatché, Florence Mousson, Petra de Graaf, Michal Mokry, Victor Guryev, H.Th. Marc Timmers, Edwin Cuppen and Ritsert C. Jansen (2010) Comparing genome-wide chromatin profiles using ChIP-chip or ChIP-seq. Bioinformatics 26:1000-1006. click here to find a pdf version

Nga-Chi Lau, Klaas W. Mulder, Arjan B. Brenkman, Shabaz Mohammed, Niels J.F. van den Broek, Albert J.R. Heck and H.Th. Marc Timmers (2010) Phosphorylation of Not4p Functions Parallel to BUR2 to Regulate Resistance to Cellular Stresses in Saccharomyces cerevisiae. PLoS One. 5e:9864. click here to find a pdf version

Laszlo Tora and H.Th. Marc Timmers (2010) The TATA box regulates TBP dynamics in vivo. TIBS 35:309-314. click here to find a pdf version

H.Th. Marc Timmers and Laszlo Tora (2010) The spectacular landscape of chromatin and NCRNAs under the Tico sunlight. EMBO Reports 3:147-149. click here to find a pdf version

W.W.M. Pim Pijnappel, Annemieke Kolkman, Marijke P.A. Baltissen, Albert J. Heck and H.Th. Marc Timmers (2009) Quantitative mass spectrometry of TATA binding protein-containing complexes and subunit phosphorylations during the cell cycle. Proteome Sci. 7:46. click here to find a pdf version

Sjoerd J.L. van Wijk and H.Th. Marc Timmers (2009) The family of Ubiquitin-Conjugating Enzymes (E2s): Deciding between Life and Death of Proteins. FASEB J. 24:09-136259. click here to find a pdf version

Nikolai Mischerikow, Gianpiero Spedale, Maarten Altelaar, H.Th.Marc Timmers, W.W.M. Pim Pijnappel and Albert J.R. Heck (2009) In-depth profiling of post-translational modifications on the related transcription factor complexes TFIID and SAGA. J.Proteome Res. 11:5020-5030. click here to find a pdf version

Folkert J. van Werven, Hetty A.A.M. van Teeffelen, Frank C.P. Holstege and H.Th. Marc Timmers (2009) Distinct promoter dynamics of the basal transcription factor TBP across the yeast genome. Nature Structural and Mol. Biol. 10:1043-1048. click here to find a pdf version

Koen M.A. Dreijerink, Radhika A. Varier, Rick van Nuland, Roel Broekhuizen,Gerlof D. Valk, Jacqueline E. van der Wal, Cornelis J.M. Lips, J. Alain Kummer and H.Th. Marc Timmers (2009) Regulation of vitamin D receptor function in MEN1-related parathyroid adenomas. Mol. Cell. Endocrinol. 313:1-8. click here to find a pdf version

Koen M.A. Dreijerink, Radhika A. Varier, Olivier van Beekum, Ellen H. Jeninga , Jo W. Höppener, Cornelis J.M. Lips, Alain J. Kummer, Eric Kalkhoven and H.Th. Marc Timmers (2009) The Multiple Endocrine Neoplasia type 1 (MEN1) tumor suppressor regulates PPAR{gamma}-dependent adipocyte differentiation. Mol. Cell. Biol. 29:5060-5069. click here to find a pdf version

Sjoerd J.L. van Wijk, Sjoerd J. de Vries, Patrick Kemmeren, Anding Huang, Rolf Boelens, Alexandre M.J.J. Bonvin and H.Th. Marc Timmers (2009) A comprehensive framework of E2-RING E3 interactions of the human ubiquitin-proteasome system. Mol. Syst. Biol. 5:295. click here to find a pdf version

Nga-Chi Lau, Annemieke Kolkman, Frederick M.A. van Schaik, Klaas W. Mulder, W.W.M. Pim Pijnappel, Albert J. Heck and H.Th. Marc Timmers (2009) Human Ccr4-Not complexes contain variable deadenylase subunits. Biochemical J. 422:443-453. click here to find a pdf version

Koen M.A. Dreijerink, Cornelis J.M. Lips and H.Th. Marc Timmers (2009) Multiple endocrine neoplasia type 1: a chromatin writer's block. J. Intern Med. 266:53-59. click here to find a pdf version

Anding Huang, Rob N.de Jong, Hans Wienk, G. Sebastiaan Winkler, H.Th. Marc Timmers and Rolf Boelens (2009) E2-c-Cbl recognition is necessary but not sufficient for ubiquitination activity. J.Mol. Biol. 385:507-519. click here to find a pdf version

Folkert J. van Werven, Harm van Bakel, Hetty van Teeffelen, Maarten Altelaar, Marian Groot Koerkamp, Albert J.R. Heck, Frank C.P. Holstege and H.Th. Marc Timmers (2008) Cooperative action of NC2 and Mot1p to regulate TATA-binding protein function across the genome. Genes Dev. 22:2359-2369. click here to find a pdf version

Hugo van Ingen, Frederik M. van Schaik, Hans Wienk, Joost Ballering, Holger Rehmann, Annemarie C. Dechesne, John A. Kruijzer, Rob M. Liskamp, H.Th. Marc Timmers and Rolf Boelens (2008) Structural insight into the recognition of the H3K4me3 mark by the TFIID subunit TAF3. Structure 16:1545-1556. click here to find a pdf version

W.W.M. Pim Pijnappel and H.Th. Marc Timmers (2008) Dubbing SAGA unveils new epigenetic crosstalk. Mol. Cell 29:152-154. click here to find a pdf version

Florence Mousson, Annemieke Kolkman, W.W.M. Pim Pijnappel, H.Th. Marc Timmers and Albert J.R. Heck (2008) Quantitative proteomics reveals regulation. Mol. Cell Proteomics. 7:845-852. click here to find a pdf version

Harm van Bakel, Folkert J. van Werven, Marijana Radonjic, Mariel O. Brok, Dik van Leenen, Frank C.P. Holstege and H.Th. Marc Timmers (2008) Improved genome-wide localization by Chip-chip using double-round T7 RNA polymerase-based amplification. Nucl. Acid. Res. 36:e21. click here to find a pdf version

Klaas W. Mulder, Akiko Inagaki, E. Cameroni , Florence Mousson, Sebastiaan G. Winkler, C. De Virgilio C, Martine A. Collart, H.Th. Marc Timmers (2007) Modulation of Ubc4p/Ubc5p-mediated stress responses by the RING-finger-dependent ubiquitin-protein ligase Not4p in Saccharomyces cerevisiae. Genetics 176:181-192. click here to find a pdf version

Michiel Vermeulen, Klaas W. Mulder, Sergei Denissov, W.W.M.Pim Pijnappel, Frederik M.A. van Schaik, Radhika A. Warrier, Marijke P.A. Baltissen, Henk G. Stunnenberg, Matthias Mann and H.Th. Marc Timmers (2007) Selective anchoring of TFIID to nucleosomes by trimethylation of histone H3 lysine four. Cell 131:58-69. click here to find a pdf version

Klaas W. Mulder, Arjan B. Brenkman, Akiko Inagaki, Niels J.F. van den Broek and H.Th. Marc Timmers (2007) Regulation of histone H3K4 tri-methylation and PAF complex recruitment by the Ccr4-Not complex. Nucleic Acids Res. 35:2428-2439. click here to find a pdf version

Sandrine Jayne, Carin G.M. Zwartjes, Frederik M.A. van Schaik, H.Th. Marc Timmers (2006) Involvement of the SMRT/NCoR-HDAC3 complex in transcriptional repression by the CNOT2 subunit of the human Ccr4-Not complex. Biochemical J. 398:461-467. click here to find a pdf version

G.Sebastiaan Winkler, Klaas W. Mulder, Vivian J. Bardwell, Eric Kalkhoven and H.Th.Marc Timmers (2006) Human Ccr4-Not complex is a ligand-dependent repressor of nuclear receptor-mediated transcription. EMBO J. 25:3089-3099. click here to find a pdf version

Koen M.A Dreijerink, Jo W.M. Höppener, H.Th. Marc Timmers and Cornelis J.M. Lips (2006) Mechanisms of Disease: multiple endocrine neoplasia type 1 relation to chromatin modifications and transcription regulation. Nat.Clin. Pract. Endocrinol. Metab. 2:562-570. click here to find a pdf version

Koen M.A. Dreijerink, Klaas W. Mulder, G. Sebastiaan Winkler, Jo W.M. Höppener, Cornelis J.M. Lips and H.Th. Marc Timmers (2006) Menin links estrogen receptor activation to histone H3K4 trimethylation. Cancer Research 66:4929-4935. click here to find a pdf version

Folkert J. van Werven and H.Th. Marc Timmers (2006) The use of biotin-tagging in Saccharomyces cerevisiae to improve the sensitivity of chromatin immunoprecipitation. Nucleic Acids Res. 34: e33. click here to find a pdf version
BirA/Avitag/SH47 Sequences

Chris J.C. van Oevelen, Hetty A.A.M. van Teeffelen, Folkert J. van Werven and H.Th.Marc Timmers (2006) Snf1p dependent SAGA recruitment and chromatin remodeling activities on the HXT2 and HXT4 promoters. J. Biol. Chem. 281:4523-4531. click here to find a pdf version

Klaas W. Mulder, G.Sebastiaan Winkler and H.Th. Marc Timmers (2005) DNA damage and replication stress induced transcription of RNR genes is dependent on the Ccr4-Not complex. Nucleic Acids Res. 33:6384-6392. click here to find a pdf version

G.Sebastiaan Winkler and H.Th. Marc Timmers (2005) Structure-Based Approaches to Create New E2-E3 Enzyme Pairs. Methods Enzymol. 399:355-366. click here to find a pdf version

Marcin P. Klejman, Xuemei Zhao, Frederik M.A. van Schaik, Winship Herr and H.Th. Marc Timmers (2005) Mutational analysis of BTAF1-TBP interaction: BTAF1 can rescue DNA-binding defective TBP mutants. Nucleic Acids Res. 33:1-11. click here to find a pdf version

Klaartje Houben, Emeric Wasielewski, Cyril Dominguez, Esther Kellenberger, R. Andrew Atkinson, H. Th. Marc Timmers, Bruno Kieffer and Rolf Boelens (2005) Dynamics and metal exchange properties of C4C4 RING domains from CNOT4 and the p44 subunit of TFIIH. J. Mol. Biol. 349:621-637. click here to find a pdf version

Marcin P. Klejman, Lloyd A. Pereira, Hester J.T. van Zeeburg, Siv Gilfillan, Michael Meisterernst and H.Th. Marc Timmers (2004) NC2alpha interacts with BTAF1 and stimulates its ATP-dependent association with TBP. Mol. Cell. Biol. 24:10072-10082. click here to find a pdf version

G. Sebastiaan Winkler, Thomas K. Albert, Cyril Dominguez, Yvonne I.A. Legtenberg, Rolf Boelens and H.Th. Marc Timmers (2004) An Altered-Specificity Ubiquitin-Conjugating Enzyme/Ubiquitin-Protein Ligase Pair. J. Mol. Biol. 337:157-165. click here to find a pdf version

Cyril Dominguez, Alexandre M.J.J. Bonvin, G. Sebastiaan Winker, Frederik M.A. van Schaik, H.Th. Marc Timmers and Rolf Boelens (2004) Structural model of the UbcH5B/CNOT4 complex revealed by combining NMR and docking approaches.Structure 12:633-644. click here to find a pdf version

Carin G.M. Zwartjes, Sandrine Jayne, Debbie L.C. van den Berg and H.Th. Marc Timmers (2004) Repression of promoter activity by CNOT2, a subunit of the transcription regulatory Ccr4-Not complex. J. Biol. Chem. 279:10848-10854. click here to find a pdf version

Lloyd A. Pereira, Marcin P. Klejman, Christine Ruhlmann, Francois Kavelaars, Mustapha Oulad-Abdelghani, H.Th. Marc Timmers and Patrick Schultz (2004) Molecular architecture of the basal transcription factor B-TFIID. J. Biol. Chem. 279:21802-21807. click here to find a pdf version

Martine A. Collart and H.Th. Marc Timmers (2004) The eukaryotic Ccr4-Not complex: a regulatory platform integrating mRNA metabolism with cellular signalling pathways? Prog. Nucleic Acid Res. Mol. Biol. 77:289-322. click here to find a pdf version

Lloyd A. Pereira, Marcin P. Klejman and H.Th. Marc Timmers (2003) Roles for BTAF1 and Mot1p in dynamics of TATA-binding protein and regulation of RNA polymerase II transcription. Gene 315:1-13. Alignments BTAF1 orthologs. click here to find a pdf version

Jean-Christophe Andrau, Chris J.C. van Oevelen, Hetty A.A.M. van Teffelen, P.A. Weil, Frank.C.P. Holstege, H.Th. Marc Timmers (2002) Mot1p is essential for TBP recruitment to selected promoters during in vivo gene activation. EMBO J. 21:5173-5183. click here to find a pdf version

H.Th. Marc Timmers (2002) Linking activators and basals in transcription. It is all in one family. Mol. Cell. 9:697-698. click here to find a pdf version

Robert Sijbrandi, Ulrike Fiedler, H.Th. Marc Timmers (2002) RNA polymerase II complexes in the very early phase of transcription are not susceptible to TFIIS-induced exonucleolytic cleavage. Nucleic Acids Res. 30:2290-2298. click here to find a pdf version

Thomas K. Albert, Hiroyuki Hanzawa, Yvonne I.A. Legtenberg, Marjolein J. de Ruwe, F.A.J. van den Heuvel, Martine A. Collart, Rolf Boelens and H.Th. Marc Timmers (2002) Identification of a ubiquitin-protein ligase subunit within the CCR4-NOT transcription repressor complex. EMBO J. 21:355-364. click here to find a pdf version

Lloyd A. Pereira, Johan A. van der Knaap, Vincent van den Boom, F. van den Heuvel, and H.Th. Marc Timmers (2001) TAFII170 interacts with the concave surface of TATA-binding protein to inhibit its DNA binding activity. Mol. Cell. Biol. 21:7523-7534. click here to find a pdf version

G. Mittler, E. Kremmer, H.Th. Marc Timmers and Michael Meisterernst (2001) Novel critical role of a human Mediator complex for basal transcription by RNA polymerase II. EMBO Reports 2:808-813. click here to find a pdf version

Ulrike Fiedler and H.Th. Marc Timmers (2001) Analysis of the open region of RNA polymerase II transcription complexes in the early phase of elongation. Nucleic Acids Res. 29:2706-2714. click here to find a pdf version

Hiroyuki Hanzawa, Marjolein J. de Ruwe, Thomas K. Albert, Peter C. van der Vliet, H.Th. Marc Timmers and Rolf Boelens (2001) The structure of the C4C4 RING finger of human NOT4 reveals features distinct from C3HC4 RING fingers. J. Biol. Chem. 276:10185-10190. click here to find a pdf version

Thomas K. Albert, M. Lemaire, Nynke van Berkum, R. Gentz, Martine A. Collart, and H.Th. Marc Timmers (2000) Isolation and characterization of human orthologs of yeast CCR4-NOT complex subunits. Nucleic Acids Res., 28:809-817. click here to find a pdf version
Supplementary information: Alignments

Ulrike Fiedler and H.Th. Marc Timmers (2000) Peeling by binding or twisting by cranking: models for promoter opening and transcription initation by RNA polymerase II. BioEssays 22:316-326. click here to find a pdf version

G. Sebastiaan Winkler, S.F. Araujo, U. Fiedler, W. Vermeulen, F. Coin, J.-M. Egly, J.H.J. Hoeijmakers, R.D. Wood, H.Th. Marc Timmers, and G. Weeda. (2000) TFIIH with inactive XPD helicase functions in transcription initiation but is defective in DNA repair. J. Biol. Chem. 275:4258-4266. click here to find a pdf version

Johan A. van der Knaap, Vincent van den Boom, J. Kuipers, M.J.T. van Eijk, Peter C. van der Vliet, and H.Th. Marc Timmers (2000) The gene for human TATA-associated factor (TAFII) 170: structure, promoter and chromosomal localization. Biochem. J., 345:521-527. click here to find a pdf version

F. Persichetti, F. Trettel, C.C. Huang, C. Fraefel, H.Th. Marc Timmers, J.W. Gusella, and M.E. MacDonald (1999) Mutant huntingtin forms in vivo complexes with distinct context-dependent conformations of the polyglutamine segment. Neurobiol. Dis. 5:364-375. click here to find a pdf version

S. Werten, G. Stelzer, A. Goppelt, F.M. Langen, P. Gros, H.Th. Marc Timmers, P.C. van der Vliet, and M. Meisterernst (1998) Interaction of PC4 with melted DNA inhibits transcription. EMBO J. 17:5103-5111. click here to find a pdf version

A.J. Marian Walhout, Peter C. van der Vliet and H.Th. Marc Timmers (1998) Sequences flanking the E-box contribute to cooperative binding by c-Myc/Max heterodimers to adjacent binding sites. Biochim. Biophys. Acta 1397:189-201. click here to find a pdf verson

S. Werten, F.W.M. Langen, R. van Schaik, H.Th. Marc Timmers, M. Meisterernst and P.C. van der Vliet (1998) High-affinity DNA binding by the C-terminal domain of the transcriptional coactivator PC4 requires simultaneous interaction with two opposing unpaired strands and results in helix destabilization. J. Mol. Biol. 276:376-377. click here to find a pdf version

Johan A. van der Knaap, J.W. Borst, Peter C. van der Vliet, R. Gentz and H.Th. Marc Timmers (1997) Cloning of the cDNA for the TATA-binding protein-associated factor (II)170 subunit of transcription factor B-TFIID reveals homology to global transcription regulators in yeast and Drosophila. Proc. Natl. Acad. Sci. USA 94:11827-11832. click here to find a pdf version

Frank C.P. Holstege, Ulrike Fiedler and H.Th. Marc Timmers (1997) Three transitions in the RNA polymerase II-transcription complex during initiation. EMBO J. 16:7468-7480. click here to find a pdf version

Frank C.P. Holstege and H.Th. Marc Timmers (1997) Analysis of open complex formation during RNA polymerase II transcription initiation using heteroduplex templates and potassium permanganate probing. Methods 12:203-211. click here to find a pdf version

A.J.M. Walhout, J.M. Gubbels, R. Bernards, P.C. van der Vliet and H.Th. Marc Timmers (1997) c-Myc/Max heterodimers bind cooperatively to the E-box sequences located in the first intron of the rat ornithine decarboxylase (ODC) enhancer. Nucleic Acids Res. 25:1493-1501. click here to find a pdf version

Frank C.P. Holstege, Peter C. van der Vliet and H.Th. Marc Timmers (1996) Opening of an RNA polymerase II promoter occurs in two distinct steps and requires the basal transcription factors IIE and IIH, EMBO J. 15:1666-1677. click here to find a pdf version

Frank C.P. Holstege, D. Tantin, M. Carey, Peter C. van der Vliet and H.Th. Marc Timmers (1995) The requirement for the basal transcription factor IIE is determined by the helical stability of promoter DNA. EMBO J. 14:810-819. click here to find a pdf version

D.C. Zijderveld, F. d'Adda di Fagagna, M. Giacca, H.Th. Marc Timmers, and Van der Vliet, P.C. (1994) Stimulation of the adenovirus major-late promoter in vitro by transcription factor USF is enhanced by the adenovirus DNA binding protein. J. Virol. 68:8288-8295. click here to find a pdf version

H.Th. Marc Timmers (1994) Transcription initiation by RNA Polymerase II does not require hydrolysis of the b-g phosphoanhydride bond of ATP. EMBO J. 13:391-399. click here to find a pdf version

F.C.P. Holstege, Peter C. van der Vliet and H.Th. Marc Timmers (1994) Instability of human TATA-binding protein CAG triplet repeats during amplification by PCR. Biochim. Biophys. Acta 1219:157-159. click here to find a pdf version

G. Hateboer, H.Th. Marc Timmers, A.K. Rustgi, M. Billaud, L.J. van 't Veer, and R. Bernards (1993) TATA-binding protein and the retinoblastoma gene product bind to overlapping epitopes on the c-myc and adenovirus E1A protein. Proc. Natl. Acad. Sci. USA 90:8489-8493. click here to find a pdf version

J.D. Parvin, H.Th. Marc Timmers, and Phil A. Sharp (1992) Promoter specificity of basal transcription factors. Cell 68:1135-1144. click here to find a pdf version

D.A. Haber, H.Th. Marc Timmers, J. Pelletier, Phil A. Sharp and D.E. Housman. (1992) A dominant mutation in the Wilms' tumor gene WT1 cooperates with the viral oncogene E1A in transformation of primary kidney cells. Proc. Natl. Acad. Sci. USA 89:6010-6014. click here to find a pdf version

H.Th. Marc Timmers, R.E.M. Meyers, and Phil A. Sharp. (1992) Composition of the transcription factor B-TFIID. Proc. Natl. Acad. Sci. USA 89:8140-8144. click here to find a pdf version 

H.Th. Marc Timmers and Phil A. Sharp. (1991) The mammalian TFIID protein is present in two functionally distinct complexes. Genes Dev. 5:1946-1956. click here to find a pdf version


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