Kops Group

Selected Publications 2008-2015

Hiruma, Y, Sacristan C, Pachis ST, Adamopoulos A, Kuijt T, Ubbink M, Von Castelmur E, Perrakis A, Kops GJPL. Competition between MPS1 and microtubules at kinetochores regulates spindle checkpoint signaling. Science 348 (2015), 1264-1267.

Drost J, Van Jaarsveld RH, Ponsioen B, Zimberlin C, Van Boxtel R, Buijs A, Sachs N, Overmeer RM, Offerhaus GJ, Schwank G, Begthel H, Korving J, Logtenberg M, Cuppen E, Snippert HJ, Medema JP, Kops GJPL, Clevers H. Sequential cancer mutations in cultured human intestinal stem cells. Nature 521 (2015), 43-7.

Vleugel M, Omerzu M, Groenewold V, Hadders MA, Lens SM, Kops GJPL. Sequential multisite phospho-regulation of KNL1-BUB3 interfaces at mitotic kinetochores. Mol Cell 57 (2015), 824-35.

Nijenhuis W, Vallardi G, Van den Dikkenberg A, Kops GJPL*, Saurin AT*. Negative feedback at kinetochores underlies a responsive spindle checkpoint signal. Nat Cell Biol. 16 (2014), 1257-64.*equal contribution and correspondence.

Jelluma N and Kops GJPL. Collateral genome instability by DNA damage in mitosis. Cancer Discov. 4 (2014), 1256-8.

Sacristan C, Kops GJPL. Joined at the hip: kinetochores, microtubules, and spindle assembly checkpoint signaling. Trends Cell Biol 25 (2014), 21-8.

Vleugel M, Tromer E, Omerzu M, Groenewold V, Nijenhuis W, Snel B, Kops GJ. Arrayed BUB recruitment modules in the kinetochore scaffold KNL1 promote accurate chromosome segregation. J Cell Biol.203 (2013), 943-55.

Nijenhuis W, Von Castelmur E, Littler D, De Marco V, Tromer E, Vleugel M, Van Osch MHJ, Snel B, Perrakis A, Kops GJPL. A TPR domain-containing N-terminal module of MPS1 is required for its kinetochore localization by Aurora B. J Cell Biol 201 (2013), 217-231.

Suijkerbuijk SJE, Vleugel M, Teixeira A, Kops GJPL. Integration of kinase and phosphatase activities by BUBR1 ensures formation of stable kinetochore-microtubule attachments. Dev Cell 23 (2012), 745-55.

Vleugel M, Hoogendoorn E, Snel B, Kops GJPL. Evolution and function of the mitotic checkpoint. Dev Cell 23 (2012), 239-50.

Suijkerbuijk SJE, Van Dam TJP, Karagöz GE, Von Castelmur E, Hubner NC, Duarte AMS, Vleugel M, Perrakis A, Rüdiger SGD, Snel B, Kops GJPL. The vertebrate mitotic checkpoint protein BUBR1 is an unusual pseudokinase. Dev Cell 22 (2012), 1321-9.

Janssen A, Van der Burg, M, Szuhai, K, Kops GJPL*, Medema RH*.Chromosome segregation errors as a cause of DNA damage and structural chromosome aberration. Science. 333 (2011), 1895-8. *corresponding authors

Saurin AT, van der Waal MS, Medema RH, Kops GJPL. Aurora B potentiates Mps1 activation to ensure rapid checkpoint establishment at the onset of mitosis. Nat Commun 2, (2011), 316.

Jelluma N, Dansen T, Sliedrecht T, Kwiatkowski NP, Kops GJPL. Release of Mps1 from kinetochores is crucial for timely anaphase onset. J Cell Biol 191 (2010), 281-290.

Janssen A, Kops GJPL*, Medema RH*. Elevating the frequency of chromosome mis-segregation as a strategy to kill tumor cells. Proc Natl Acad Sci U S A. 106 (2009) 19108-13. *equal contribution and correspondence

Jelluma N, Brenkman AB, Van den Broek NJF, Cruijsen CWA, Van Osch MHJ, Lens SMA, Medema RH and Kops GJPL. Mps1 Phosphorylates Borealin to Control Aurora B Activity and Chromosome Alignment. Cell 132 (2008). 233-246. 

Publications 2015

Hiruma, Y, Sacristan C, Pachis ST, Adamopoulos A, Kuijt T, Ubbink M, Von Castelmur E, Perrakis A, Kops GJPL. Competition between MPS1 and microtubules at kinetochores regulates spindle checkpoint signaling. Science 348 (2015), 1264-1267.

Drost J, Van Jaarsveld RH, Ponsioen B, Zimberlin C, Van Boxtel R, Buijs A, Sachs N, Overmeer RM, Offerhaus GJ, Schwank G, Begthel H, Korving J, Logtenberg M, Cuppen E, Snippert HJ, Medema JP, Kops GJPL, Clevers H. Sequential cancer mutations in cultured human intestinal stem cells. Nature 521 (2015), 43-7.

Vleugel M, Omerzu M, Groenewold V, Hadders MA, Lens SM, Kops GJPL. Sequential multisite phospho-regulation of KNL1-BUB3 interfaces at mitotic kinetochores. Mol Cell 57 (2015), 824-35.

Overlack K, Primorac I, Vleugel M, Krenn V, Maffini S, Hoffmann I, Kops GJPL, Musacchio A. A molecular basis for the differential roles of Bub1 and BubR1 in the spindle assembly checkpoint. Elife 4 (2015). e05269

Sacristan C, Kops GJPL. Joined at the hip: kinetochores, microtubules, and spindle assembly checkpoint signaling. Trends Cell Biol 25 (2015), 21-8.

Publications 2014

Nijenhuis W, Vallardi G, Van den Dikkenberg A, Kops GJPL*, Saurin AT*. Negative feedback at kinetochores underlies a responsive spindle checkpoint signal. Nat Cell Biol. 16 (2014), 1257-64.*equal contribution and correspondence.

Jelluma N and Kops GJPL. Collateral genome instability by DNA damage in mitosis. Cancer Discov. 4 (2014), 1256-8.

Kloet SL, Baymaz HI, Makowski M, Groenewold V, Jansen PW, Berendsen M, Niazi H, Kops GJPL, Vermeulen M. Towards elucidating the stability, dynamics and architecture of the nucleosome remodeling and deacetylase complex by using quantitative interaction proteomics. FEBS J. (2014) in press.

Shaltiel IA, Aprelia M, Saurin AT, Chowdhury D, Kops GJPL, Voest EE, Medema RH. Distinct phosphatases antagonize the p53 response in different phases of the cell cycle. Proc Natl Acad Sci USA. 111 (2014), 7313-8.

Kuijt TE, Omerzu M, Saurin AT, Kops GJPL. Conditional targeting of MAD1 to kinetochores is sufficient to reactivate the spindle assembly checkpoint in metaphase. Chromosoma 123 (2014) 471-80.

Kops GJPL. Cell division: SACing the anaphase problem. Curr Biol. 24 (2014), R224-6.

Akopyan K, Silva Cascales H, Hukasova E, Saurin AT, Müllers E, Jaiswal H, Hollman DA, Kops GJPL, Medema RH, Lindqvist A. Assessing kinetics from fixed cells reveals activation of the mitotic entry network at the S/G2 transition. Mol Cell. 53 (2014), 843-53.

Publications 2013

Vleugel M, Tromer E, Omerzu M, Groenewold V, Nijenhuis W, Snel B, Kops GJ. Arrayed BUB recruitment modules in the kinetochore scaffold KNL1 promote accurate chromosome segregation. J Cell Biol. 203 (2013), 943-55. (link to paper)

van de Pasch LA, Miles AJ, Nijenhuis W, Brabers NA, van Leenen D, Lijnzaad P, Brown MK, Ouellet J, Barral Y, Kops GJ, Holstege FC. Centromere binding and a conserved role in chromosome stability for SUMO-dependent ubiquitin ligases. PLoS One 8 (2013), e65628

Earnshaw WC, Allshire RC, Black BE, Bloom K, Brinkley BR, Brown W, Cheeseman IM, Choo KH, Copenhaver GP, Deluca JG, Desai A, Diekmann S, Erhardt S, Fitzgerald-Hayes M, Foltz D, Fukagawa T, Gassmann R, Gerlich DW, Glover DM, Gorbsky GJ, Harrison SC, Heun P, Hirota T, Jansen LE, Karpen G, Kops GJ, Lampson MA, Lens SM, Losada A, Luger K, Maiato H, Maddox PS, Margolis RL, Masumoto H, McAinsh AD, Mellone BG, Meraldi P, Musacchio A, Oegema K, O'Neill RJ, Salmon ED, Scott KC, Straight AF, Stukenberg PT, Sullivan BA, Sullivan KF, Sunkel CE, Swedlow JR, Walczak CE, Warburton PE, Westermann S, Willard HF, Wordeman L, Yanagida M, Yen TJ, Yoda K, Cleveland DW. Esperanto for histones: CENP-A, not CenH3, is the centromeric histone H3 variant. Chromosome Res. 21 (2013), 101-6.

Nijenhuis W, Von Castelmur E, Littler D, De Marco V, Tromer E, Vleugel M, Van Osch MHJ, Snel B, Perrakis A, Kops GJPL. A TPR domain-containing N-terminal module of MPS1 is required for its kinetochore localization by Aurora B. J Cell Biol 201 (2013), 217-231. (link to paper)

Hennrich ML, Marino F, Groenewold V, Kops GJPL, Mohammed S, Heck AJ. Universal quantitative kinase assay based on diagonal SCX chromatography and stable isotope dimethyl labeling provides high-definition kinase consensus motifs for PKA and human Mps1. J Proteome Res 12 (2013), 2214-24.

Publications 2012

Suijkerbuijk SJE, Vleugel M, Teixeira A, Kops GJPL. Integration of kinase and phosphatase activities by BUBR1 ensures formation of stable kinetochore-microtubule attachments. Dev Cell 23 (2012), 745-55. (link to paper)

Vleugel M, Hoogendoorn E, Snel B, Kops GJPL. Evolution and function of the mitotic checkpoint. Dev Cell 23 (2012), 239-50. (link to paper)

Suijkerbuijk SJE, Van Dam TJP, Karagöz GE, Von Castelmur E, Hubner NC, Duarte AMS, Vleugel M, Perrakis A, Rüdiger SGD, Snel B, Kops GJPL. The vertebrate mitotic checkpoint protein BUBR1 is an unusual pseudokinase. Dev Cell 22 (2012), 1321-9. (link to paper)

Kops GJPL, Shah JV. Connecting up and clearing out: how kinetochore attachment silences the spindle assembly checkpoint. Chromosoma, in press. DOI: 10.1007/s00412-012-0378-5 (link to paper)

Van der Waal MS, Saurin AT, Vromans MJ, Vleugel M, Wurzenberger C, Gerlich DW, Medema RH, Kops GJPL, Lens SMA. Mps1 promotes rapid centromere accumulation of Aurora B. EMBO Rep 13, 847-54. (link to paper) 

Publications 2011

Janssen A, Van der Burg, M, Szuhai, K, Kops GJPL*, Medema RH*.Chromosome segregation errors as a cause of DNA damage and structural chromosome aberration. Science. 333 (2011), 1895-8. *corresponding authors (link to paper) 

Hennrich ML, Groenewold V, Kops GJPL, Heck AJ, Mohammed S. Improving Depth in Phosphoproteomics by Using a Strong Cation Exchange-Weak Anion Exchange-Reversed Phase Multidimensional Separation Approach. Anal Chem. 83 (2011), 7137-43. (link to paper)

Saurin AT, van der Waal MS, Medema RH, Kops GJPL. Aurora B potentiates Mps1 activation to ensure rapid checkpoint establishment at the onset of mitosis. Nat Commun 2, (2011), 316. (link to paper)

Avo Santos M, van de Werken C, de Vries M, Jahr H, Vromans M, Laven JS, Fauser BC, Kops GJPL, Lens SMA, Baart EB. A role for Aurora C in the chromosomal passenger complex during human preimplantation embryo development. Hum Reprod (2011). doi 10.1093/humrep/der111(link to paper)

Janssen A, Kops GJPL, Medema RH. Targeting the mitotic checkpoint to kill tumor cells. Horm Cancer 2 (2011), 113-6. (link to paper)

Varier RA, Outchkourov NS, de Graaf P, van Schaik FM, Ensing HJ, Wang F, Higgins JM, Kops GJPL, Timmers HT. A phospho/methyl switch at histone H3 regulates TFIID association with mitotic chromosomes. EMBO J 29 (2011), 3967-78. (link to paper)

Publications 2010

Jelluma N, Dansen T, Sliedrecht T, Kwiatkowski NP, Kops GJPL. Release of Mps1 from kinetochores is crucial for timely anaphase onset. J Cell Biol 191 (2010), 281-290. (link to paper)

Suijkerbuijk SJE, Van Osch MHJ, Bos FL, Hanks S, Rahman N, Kops GJPL. Molecular causes for BUBR1 dysfunction in the human cancer predisposition syndrome mosaic variegated aneuploidy. Cancer Research 70 (2010), 4891-900. (link to paper)

 

Sliedrecht T, Zhang C, Shokat KM, Kops GJPL. Chemical genetic inhibition of Mps1 in stable human cell lines reveals novel aspects of Mps1 function in mitosis. PLoS ONE 5(4) (2010) e10251.(link to paper)

 

Kops GJPL*, Voet MV, Manak MS, van Osch MHJ, Naini SM, Brear A, McLeod IX, Hentschel DM, Yates JR, van den Heuvel S, Shah JV*. APC16 is a conserved subunit of the anaphase-promoting complex/cyclosome. J Cell Science 123 (2010), 1623-33. *equal contribution and correspondence (link to paper)

  

Kwiatkowski N, Jelluma N, Filippakopoulos P, Soundararajan M, Manak MS, Kwon M, Choi HG, Sim T, Deveraux QL, Rottmann S, Pellman D, Shah JV, Kops GJPL, Knapp S, Gray NS. Small-molecule kinase inhibitors provide insight into Mps1 cell cycle function. Nat Chem Biol. 6 (2010), 359-368. (link to paper)

 

Kops, GJPL*, Saurin A, Meraldi, P*. Finding the middle ground: how kinetochores power chromosome congression. Cell Mol Life Sci. (2010) doi:10.1007/s00018-010-0321-y *equal contribution and correspondence (link to paper)

  

Publications 2009

 

Janssen A, Kops GJPL*, Medema RH*. Elevating the frequency of chromosome mis-segregation as a strategy to kill tumor cells. Proc Natl Acad Sci U S A. 106 (2009) 19108-13. *equal contribution and correspondence (link to paper)

  

Publications 2008

 

Suijkerbuijk SJ and Kops GJPL. Preventing aneuploidy: The contribution of mitotic checkpoint proteins.Biochim Biophys Acta. 1786 (2008), 24-38. (link to paper)

  

Jelluma N, Brenkman AB, McLeod IX, Yates III JR, Cleveland DW, Medema RH, Kops GJPL. Chromosomal Instability by Inefficient Mps1 Auto-activation Due to a Weakened Mitotic Checkpoint and Lagging Chromosomes. PloS ONE 3(6)(2008). e2415. (link to paper)

 

Kops GJPL.The kinetochore and spindle checkpoint in mammals. Frontiers in Bioscience 13 (2008), 3606-3620. (link to paper)

  

Jelluma N, Brenkman AB, Van den Broek NJF, Cruijsen CWA, Van Osch MHJ, Lens SMA, Medema RH and Kops GJPL. Mps1 Phosphorylates Borealin to Control Aurora B Activity and Chromosome Alignment. Cell 132 (2008). 233-246. (link to paper)

 

Selected Publications 2004-2007

Kops GJPL, Weaver BAA, Cleveland DW. On the road to cancer: Aneuploidy and the mitotic checkpoint. Nature Reviews Cancer 5 (2005), 773-785. (link to paper)

  

Kops GJPL, Kim Y., Weaver BAA., Mao Y., McLeod I., Yates III JR., Tagaya M. and Cleveland DW. ZW10 links mitotic checkpoint signaling to the structural kinetochore. J Cell Biology 169 (2005), 49-60. (link to paper)

 

Kops GJPL, Foltz DF and Cleveland DW. Lethality to human cancer cells through masssive chromosome loss by inhibition of the mitotic checkpoint. Proc Natl Acad Sci USA 101 (2004), 8699-8704. (link to paper)

  

Selected Publications 1999-2002, on the PI3K-PKB-FOXO signaling pathway

Kops GJPL, Dansen TB, Polderman PE, Saarloos I, Wirtz KWA,  Coffer PJ, Huang TT, Bos JL, Medema RH and Burgering BMT. Forkhead transcription factor FOXO3a protects quiescent cells from oxidative stress. Nature 419 (2002), 316-21. (link to paper

Burgering BMT and Kops GJPL. Cell cycle and death control: Long live Forkheads. TIBS 27 (2002), 352-60. (link to paper)

  

Kops GJPL*, Medema RH*, Glassford J, Essers MA, Dijkers PF, Coffer PJ, Lam EW and Burgering BMT. Control of cell cycle exit and entry by protein kinase B-regulated Forkhead transcription factors. Mol Cell Biol 22 (2002), 2025-36. *These authors contributed equally (link to paper)

  

Brownawell AM, Kops GJPL, Macara IG and Burgering BMT. Inhibition of nuclear import by protein kinase B/Akt regulates the subcellular distribution and activity of the Forkhead transcription factor AFX.Mol Cell Biol 21 (2001), 3534-46. (link to paper)

  

Medema RH*, Kops GJPL*, Bos JL and Burgering BMT. AFX-like Forkhead transcription factors mediate cell-cycle regulation by Ras and PKB through p27kip1. Nature 404 (2000), 782-7.*These authors contributed equally (link to paper)

 

Kops GJPL, de Ruiter ND, De Vries-Smits AM, Powell DR, Bos JL and Burgering BMT. Direct control of the Forkhead transcription factor AFX by protein kinase B. Nature 398 (1999), 630-4. (link to paper)